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Dr. Libo Shan

Shan, Dr. Libo
Dr. Libo Shan
Professor, Plant Molecular Biology
132A Borlaug
Undergraduate Education
B.S.: Beijing Normal University
Graduate Education
Ph.D.: Kansas State University
Post-Doctoral: Harvard Medical School

Research Emphasis:

Plant Molecular Biology

We and plants detect “danger” by recognizing microbe-associated molecules and launch complex innate immune signaling to prevent infection. Successful pathogens, however, have evolved multiple sophisticated virulence strategies to dampen the host immunity and result in infection. My research interest is to understand the genetic, molecular and biochemical mechanisms of the dynamic host-microbe interactions using Arabidopsis-Pseudomonas as a model plant-pathogen system. My ultimate goal is to understand how the host-microbe interactions shape the evolution of microbial pathogenicity and plant immunity in both model and economically important plants.


  • Lin, W., Li, B., Lu, D., Chen, S., Zhu, N., He, P., and Shan, L., (2014) Tyrosine phosphorylation of BAK1/BIK1 mediates Arabidopsis innate immunity. Proc Natl Acad Sci U S A. (In press).
  • Zhou, J., Wu, S., Chen, X., Liu, C., Sheen, J., Shan, L., and He, P. (2013). Pseudomonas syringae Effector HopF2 Suppresses Arabidopsis Immunity by Targeting BAK1. The Plant Journal. 77:235–245.
  • Cheng C., Gao X., Feng B., Sheen J., Shan L., He P. (2013) Plant immune response to pathogens differs with changing temperatures. Nat Commun. 4:2530. doi: 10.1038/ncomms3530
  •  Lin, W., Lu, D., Gao, X., Jian, S., Ma, X., Wang, Z., Mengiste, T., He, P., and Shan L. (2013) Inverse modulation of plant immunity and plant development by a receptor-like cytoplasmic kinase BIK1. Proc Natl Acad Sci U S A110 (29):12114-9.
  • Cui, F., Wu, S., Sun, W., Coaker, G., Kunkel, B. N, Shan, L. (2013) The Pseudomonas syringae type III effector AvrRpt2 promotes pathogen virulence via stimulating Arabidopsis Auxin/Indole Acetic Acid protein turnover. Plant Physiology 162:1018-1029.
  • Gao, X., Chen, X., Lin, W., Lu, D., Niu, Y., Li, L., Cheng, C., McCormack, M., Sheen, J., Shan, L., and He, P. (2013) Bifurcation of Arabidopsis NLR Immune Signaling via Ca2+-Sensor Protein Kinases. PLoS Pathog 9, e1003127.
  • Lin, W., Ma, X., Shan, L., and He, P. (2013) Big roles of small kinases: the complex functions of receptor-like cytoplasmic kinases in plant immunity and development. Journal of Integrative Plant Biology. 55:1188-1197
  • Gao, X., Li, F., Li, M., Kianinejad, A., Dever, J., Wheeler, T., Li, Z., He, P., and Shan, L., 2013 Cotton GhBAK1 mediates Verticillium wilt resistance and cell death. Journal of Integrative Plant Biology55(7):586-96.
  • Gao X, Shan L (2013) Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing. Methods in Mol Biol 975, 157-165.
  • Lu, D., Lin, W., Gao, X., Wu, S., Cheng, C., Avila, J.,Heese, A.,Devarenne, T., He, P., and Shan, L. (2011) Direct ubiquitination of pattern recognition receptor FLS2 attenuates plant innate immunity. Science. 332: 1439-1442. Perspective: O’Neill, L., (2011) Innate immunity in plants goes to the PUB. Science 332: 1386-1387.
  • Gao, X., BrittR.C., Shan, L. and He, P. (2011) Agrobacterium-mediated virus-induced gene silencing assay in cotton. J Vis Exp. 54
  • Gao, X., Wheeler, T., Li, Z., Kenerley, C., He, P., and Shan, L. (2011) Silencing GhNDR1 and GhMKK2 compromised cotton resistance to Verticillium wiltThe Plant Journal. 66(2):293-305.
  • Wu, S., Lu, D., Kabbage, M., Wei, H., Swingle, B., Dickman, M., He, P., and Shan, L. (2011) Bacterial effector HopF2 suppresses Arabidopsis innate immunity at the plasma membrane. Mol. Plant Microbe Interact. 24(5):585-93.
  • Lu, D., Wu, S., He, P., and Shan, L. (2010) Phosphorylation of receptor-like cytoplasmic kinases by bacterial flagellin. Plant Signal Behav. 5 (5).
  • Lu, D., Wu, S., Gao, X., Zhang, Y., Shan, L. and He, P. (2010) A receptor-like cytoplasmic kinase BIK1 associates with flagellin receptor complex to initiate plant innate immunity. Proc Natl Acad Sci U S A. 107:496-501.
  • Lu, D., He, P., and Shan, L. (2010) Bacterial effectors target BAK1-associated receptor complexes: one stone two birds. Communicative & Integrative Biology. 3 (2):80-83.
  • Boudsocq, M., WillmannM. R., McCormack, M., Lee, H., Shan L., He P., Bush, J., Cheng, S., and  Sheen J., (2010) Differential innate immune signalling via Ca2+ sensor protein kinases. Nature. 464: 418-422.
  • Chinchilla, D., Shan, L., He, P., de Vries, S. and Kemmerling, B. (2009) One for all-the receptor-associated kinase BAK1. Trend in Plant Science.14 (10) 535-541.
  • Shan, L, He, P, Li, J., Heese, A., Peck, S. C., Nurnberger, T., Martin, G. B., and Sheen, J (2008). Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host & Microbe 4: 17-27.
  • Chen, Z., Agnew, J., Cohen, J., He, P., Shan, L., Sheen, J., and Kunkel, K. (2007). The Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology. Proc. Natl. Acad. Sci. U. S. A 104: 20131-20136.
  • Shan, L., He, P., and Sheen, J. (2007). Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host & Microbe 1: 167-174.
  • He, P., Shan, L., and Sheen, J. (2007) Elicitation and suppression of MAMP-triggered immunity in plant-microbe interactions. Cell Microbiol 9: 1385-1396.
  • Shan, L., He, P., and Sheen, J. (2007) Endless hide-and-seek: dynamic coevolution in plant bacterium warfare. Journal of Integrative Plant Biology 49:105-111.
  • He, P., Shan, L. and Sheen, J. (2006) The use of protoplasts to study innate immune responses. Methods in Mol Biol. 354: 1-10.
  • He, P.*, Shan, L.*, Lin, N.-C., Martin, G., Kemmerling, B., Nurnberger, T., and Sheen, J. (2006). Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity. Cell 125: 563-575. (*Co-first author)
  • Robert-Seilaniantz, A.*, Shan, L.*, Zhou, J.-M., and Tang, X. (2006). The Pseudomonas syringae pv. tomato DC3000 type III effector HopF2 has a putative myristoylation site required for its avirulence and virulence functions. Mol. Plant Microbe Interact. 19: 130-138. (*Co-first author)
  • Shan, L., Oh, H., Chen, J., Guo, M., Zhou, J.-M., Alfano, J.R., Collmer, A., Jia, X., and Tang X. (2004). The HopPtoF locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector. Mol. Plant Microbe Interact. 17: 447-455.
  • Li, J., Shan, L., Zhao, T., Zhou, J.-M., and Tang, X. (2002). Salicylic acid and ethylene are not essential for spontaneous cell death and PR gene induction in tomato plants overexpressing Pto. Mol. Plant Microbe Interact. 15: 654-661.
  • Petnicki-Ocwieja, T., Schneider, D. J., Tam, V. C., Chancey, S. T., Shan, L., Jamir, Y., Schechter, L. M., Janes, M. D., Buell, C. R., Tang, X., et al. (2002).Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. U S A 99: 7652-7657.
  • He, P., Warren, R. F., Zhao, T., Shan, L., Zhu, L., Tang, X., and Zhou, J.-M. (2001). Overexpression of Pti5 in tomato potentiates pathogen-induced defense gene expression and enhances disease resistance to Pseudomonas syringae pv. tomato. Mol. Plant Microbe Interact. 14: 1453-1457.
  • Shan, L., Thara, V.K., Martin, G.B., Zhou, J.-M., and Tang, X. (2000). The Pseudomonas AvrPto protein is differentially recognized by tomato and tobacco and is localized to the plant plasma membrane. Plant Cell 12: 2323-2338.
  • Shan, L., He, P., Zhou, J.-M., and Tang, X. (2000). A cluster of mutations disrupt the avirulence but not the virulence function of AvrPto. Mol. Plant Microbe Interact. 13: 592-598.
  • Shan, L., Li, Y.W., Zhao, T.H., Liang, H., Ouyang, J.W., Jia, S.E., and Jia, X. (2000). Estimation of biolistic transformation effect by transient expression of C1-R regulatory genes of anthocyanin biosynthesis. Acta Genetica Sinica 27: 65-69.