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Dr. Daniel J. Ebbole

Ebbole, Dr. Daniel J.
Dr. Daniel J. Ebbole
Professor, Fungal Development & Pathogenesis
Office:
321B LF Peterson
Email:
Phone:
979-845-4831
Undergraduate Education
B.S. Biochemistry, Indiana University, 1983
B.A. Microbiology, Indiana University, 1983
Graduate Education
Ph.D. Biochemistry, Purdue University, 1988
Courses Taught
PLPA 607 Pathogen Strategies in Plant Disease
BESC403 Sampling and Environmental Monitoring
BESC481 Environmental Issues of the US and China
BESC489 Study Abroad: International Perspectives on Environmental Issues

Research Emphasis

Development and pathogenesis share the common features of responding to environmental conditions to execute a program of gene expression resulting in new cell types.  During asexual sporulation in fungi (conidiation) pathways involving several transcription factors control the temporal and spatial patterns of gene expression. Genomic resources are helping to define the regulatory pathways controlling conidiation and the role of cell type-specific gene in morphogenesis in several fungal systems, including Neurospora crassa and Aspergillus nidulans. A question my lab is exploring is whether conidiation pathways have evolved independently (convergent evolution) or if conidiation arose from an ancient pathway (divergent evolution) in different fungal lineages.  In either case, the process of answering this question will uncover the mechanism for creation of new transcriptional circuits that control developmental processes, providing an understanding of how pathways controlling development and pathogenesis evolve.  The Ebbole Lab participates in the Program for the Biology of Filamentous Fungi group.

An important question in plant pathogenesis is to understanding the functions of pathogen effectors and their host target(s).   Fungal effectors play roles in suppressing host defense mechanisms, however, other biotrophic functions, such as manipulating host physiology to promote nutrient acquisition and cell-to-cell movement are possible.  Therefore, identification of the full set of fungal proteins secreted during host invasion is a major effort in plant pathology research.  Candidate effectors are generally identified by virtue of i) their expression in planta ii) assessing their activity on the host using purified proteins or by manipulating expression iii) detecting the rapid evolution of effector genes due to selective pressure from the host.  My lab is using a combination of these approaches to identify and characterize a gene family of putative effectors from Magnaporthe oryzae, the rice blast fungus.

Publications

Chen S, Songkumarn P, Venu RC, Bellizi M, Hu J, Liu W, Ebbole D, Meyers B, Mitchell T, Wang GL. 2013. Identification and characterization of in planta-expressed secreted effector proteins from Magnaporthe oryzae that induce cell death in rice. Mol. Plant-Microbe Interact. 26:191-202.

Kubicek CP, Herrera-Estrella A, Seidl-Seiboth V, Martinez DA, Druzhinina IS, Thon M, Zeilinger S, Casas-Flores S, Horwitz BA, Mukherjee PK, Mukherjee M, Kredics L, Alcaraz LD, Aerts A, Antal Z, Atanasova L, Cervantes-Badillo MG, Challacombe J, Chertkov O, McCluskey K, Coulpier F, Deshpande N, von Doehren H, Ebbole DJ, Esquivel-Naranjo EU, Fekete E, Flipphi M, Glaser F, Gomez-Rodriguez EY, Gruber S, Han C, Henrissat B, Hermosa R, Hernandez-Onate M, Karaffa L, Kosti I, Le Crom S, Lindquist E, Lucas S, Lubeck M, Lubeck PS, Margeot A, Metz B, Misra M, Nevalainen H, Omann M, Packer N, Perrone G, Uresti-Rivera EE, Salamov A, Schmoll M, Seiboth B, Shapiro H, Sukno S, Tamayo-Ramos JA, Tisch D, Wiest A, Wilkinson HH, Zhang M, Coutinho PM, Kenerley CM, Monte E, Baker SE, Grigoriev IV.  2011. Comparative genome sequence analysis underscores mycoparasitism as the ancestral life style of Trichoderma. Genome Biol. 12:R40.

Chung DW, Greenwald C, Upadhyay S, Ding S, Wilkinson HH, Ebbole DJ, Shaw BD. 2011. acon-3, the Neurospora crassa ortholog of the developmental modifier, medA, complements the conidiation defect of the Aspergillus nidulans mutant. Fungal Genet. Biol. 48:370-376.

Greenwald, CJ., Kasuga, T., Glass, NL., Shaw, BD., Ebbole, DJ., Wilkinson, HH. 2010. Temporal and Spatial Regulation of Gene Expression During Asexual Development in Neurospora crassa. Genetics. 186:1217-1230.

Zhou J, Lin CZ, Zheng XZ, Lin XJ, Sang, SH., Wang ZH., Ebbole D.J., Lu GD. 2009. Functional analsyis of an a-1,2-mannosidase from Magnaporthe oryzae. Curr. Genet. 55:485-496.

Meng S, Brown DE, Ebbole DJ, Torto-Alalibo T, Oh YY, Deng J, Mitchell TK, Dean RA. 2009. Gene Ontology annotation of the rice blast fungus, Magnaporthe oryzae. BMC Microbiol. 19:S8.

Books/Reviews

Cellular and Molecular Biology of Filamentous Fungi.  ASM Press.  2010. Katherine Borkovich and Daniel J. Ebbole, eds. Washington DC. 788 pages.

Ebbole D.J. 2007. Magnaporthe as a model for understanding host-pathogen interactions. Annu. Rev. Phytopathol. 45:437-456.