i) Identify differentially expressed sequences using Suppressive Subtractive Hybridization technique followed by designing sequence specific probes and primers for sorghum downy mildew disease and screening sorghum cultivars/elite lines using Real-Time RT-PCR

ii) Microsatellites Capturing from fungal genomes of sorghum downy mildew, head smut and anthracnose and their molecular characterization, genetic structure and phylogenetic analyses on diversified isolates.

iii) Molecular marker analysis (AFLP & SSRs) using high throughput LICOR automated sequencing system for the identification gene(s) for head smut and anthracnose disease resistance in sorghum.

iv) SNPs discovery and haplotypte analysis by utilizing Ecotilling with PCR-amplified resistance gene analogs in high sorghum biomass germplasm accessions for anthracnose fungal disease resistance.