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| Search | Home Page >Directory >Faculty : Current Page |
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| Herman B. Scholthof Professor |
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Office: 413D LF Peterson Phone: 979-862-1495 Email: herscho@tamu.edu Lab Web Page: http://plantpathology.tamu.edu/LabWebPages/Scholthof/index.html |
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| Education | ||
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Ph.D. Plant Pathology (1990) University of Kentucky |
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| Research Emphasis: | ||
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Plant Virology In our Plant Virology laboratory we study the molecular mechanisms that determine whether a plant is susceptible or resistant to infection with a virus. Although there are multiple factors that influence the ability of a virus to infect a plant, crucial events are viral movement and the evasion of resistance responses. To investigate some of these aspects we use Tomato bushy stunt virus (TBSV) as a model system. TBSV has an RNA genome with five genes of which two encode proteins, P22 and P19, that are involved in virus spread and which can also act as elicitors of resistance responses. P22 is required for cell-to-cell movement and P19 performs host-specific activities for virus transport and it is a suppressor of defense-related gene silencing. We are currently conducting experiments to examine the biochemical properties of P22 and P19 and to examine their interaction with host proteins. A separate research project involves a molecular characterization of a newly recognized virus on wheat and corn that is transmitted by wheat curl mites. |
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| Teaching | ||
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PLPA 616: Methods in molecular biology of plant-microbe interactions. (2 hr) The topics of this course focus on the concepts and techniques used in molecular plant pathology to study the interactions between hosts and pathogens. The focus will be on understanding the rationale for implementing certain procedures and the theoretical concepts underlying the methodology. PLPA 620: Plant virology. (3 hr with lab). An overview of plant virology with an emphasis on the molecular biology of virus-host interactions; topics include virus replication, gene expression, movement, symptom induction, transmission, control, and topics from current literature. The laboratory focuses on virus inoculation, purification, and molecular diagnostics. PLPA 689: Special Topics
in Virology: Viral Vectors and Gene Therapy. (3 hr.). Viruses are proving
to be exceptional vehicles for the expression of recombinant protein.
This course will describe several viral vector systems, their development
and uses as research tools and for gene therapy. |
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| Recent Publications | ||
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Omarov, R., Ciomperlik, J., and Scholthof, H.B. (2007). RNA-iassociated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex. Proc. Natl. Acad. Sci. USA (in press). Scholthof, H.B. (2006). The Tombusvirus-encoded P19: from irrelevance to elegance. Nat. Rev. Microbiol. 4:405-411. Omarov, R., Sparks, K., Smith, L., Zindovic, J., and Scholthof, H.B. (2006). Biological relevance of a stable biochemical interaction between the tombusvirus-encoded P19 and siRNAs. J. Virol. 80:3000-3008. Skare, J.M., Wijkamp, I., Denham, I., Rezende, J.A.M., Kitajima, E.W., Park, J.W., Desvoyes, B., Rush, C.M., Michels, G., Scholthof, K.-B. G., and Scholthof, H.B. (2006). A new eriophyid mite-borne membrane-enveloped virus complex isolated from plants. Virology 347:343-353. Scholthof H.B. (2005). Plant virus transport: motions of functional equivalence. Trends in Plant Science 10:376-382. Yamamura, Y., and Scholthof, H.B. (2005). Pathogen profile: Tomato bushy stunt virus: A resilient model system for studying virus-plant interactions. Molec. Plant Pathol. 6:491-502 Park, J.-W., Faure-Rabasse, S., Robinson, M.A., Desvoyes, B., and Scholthof, H.B. (2004). The multifunctional plant viral suppressor of gene silencing P19 interacts with itself and an RNA binding host protein. Virology 323:49-58. Omarov, R.T. Rezende, J., and Scholthof, H.B. (2004). Host-specific generation and maintenance of Tomato bushy stunt virus defective interfering RNAs. Molec. Plant Microbe Interact. 17:195-201. Turina, M., Omarov, R., Murphy, J.F., Bazaldua-Hernandez, C., Desvoyes, B., and Scholthof, H.B (2003). A newly identified role for Tomato bushy stunt virus P19 in short distance spread. Molec. Plant Pathol. 4:67-72 Skare, J. M, Wijkamp, I., Rezende, J., Michels G., Rush, C., Scholthof, K.-B. G., and Scholthof, H.B. (2003). Colony establishment and maintenance of the eriophyid wheat curl mite Aceria tosichella for controlled transmission studies on a new virus-like pathogen. J. Virol. Meth. 108:133-137. |
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